LOCUS       pLV-[hsa-miR-106b]        9136 bp    DNA     circular     12-APR-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7057..7917
                     /label=Amp(R)
     promoter        8118..8658
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5662..5896
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6471..6926)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5968..6098
                     /label=SV40 pA
     misc_feature    6099..6312
                     /label=SV40 ori
     misc_feature    4983..5579
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3601
                     /label=intron
     CDS             3648..4973
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3391
                     /label=hsa-mir-106b precursor
BASE COUNT     2143 a      2392 c      2483 g      2118 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcct ggtaagtgcc 
     3121 caaattgctg gagggccatc tgttttgacc cttaaagggg tagctcctta ccgtgctctc 
     3181 attgccgcct ccccacctcc cgctccagcc ctgccggggc taaagtgctg acagtgcaga 
     3241 tagtggtcct ctccgtgcta ccgcactgtg ggtacttgct gctccagcag ggcacgcaca 
     3301 gcgtccgtgg agggaaaggc cttttcccca cttcttaacc ttcactgaga gggtggttgg 
     3361 ggtctgtttc actccatgtg tcctagatcc tgctagctcg agcttttgga gtacgtcgtc 
     3421 tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac 
     3481 tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt 
     3541 tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca 
     3601 ggtgtcgtga aaactacccc tctagagtcg agctaccggt cgccaccatg gtgagcaagg 
     3661 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg 
     3721 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg 
     3781 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca 
     3841 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc 
     3901 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg 
     3961 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct 
     4021 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga 
     4081 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg 
     4141 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca 
     4201 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg 
     4261 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc 
     4321 gccactccac cggcggcatg gacgagctgt acaaggaccc accggtcgcc accatgaccg 
     4381 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc 
     4441 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca 
     4501 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca 
     4561 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg 
     4621 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc 
     4681 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt 
     4741 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg 
     4801 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct 
     4861 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg 
     4921 agtgcccgaa ggaccgcgcg acctggtgca tgacccgcaa gcccggtgcc tgagcggccg 
     4981 cgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 
     5041 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 
     5101 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 
     5161 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 
     5221 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 
     5281 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 
     5341 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg 
     5401 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 
     5461 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 
     5521 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg 
     5581 aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac 
     5641 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg 
     5701 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc 
     5761 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 
     5821 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 
     5881 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca 
     5941 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa 
     6001 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 
     6061 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa 
     6121 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 
     6181 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 
     6241 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta 
     6301 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac 
     6361 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc 
     6421 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg 
     6481 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 
     6541 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 
     6601 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg 
     6661 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg 
     6721 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 
     6781 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt 
     6841 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt 
     6901 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc 
     6961 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 
     7021 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 
     7081 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 
     7141 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 
     7201 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 
     7261 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa 
     7321 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 
     7381 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 
     7441 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 
     7501 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 
     7561 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg 
     7621 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 
     7681 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 
     7741 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 
     7801 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 
     7861 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 
     7921 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 
     7981 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 
     8041 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 
     8101 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 
     8161 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 
     8221 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta 
     8281 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 
     8341 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 
     8401 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 
     8461 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 
     8521 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 
     8581 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 
     8641 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 
     8701 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 
     8761 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 
     8821 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct 
     8881 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 
     8941 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct 
     9001 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac 
     9061 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa 
     9121 caaaagctgg agctgc 
//
